Table 5.
Cytokine CD4+ T cell response in the lamina propria lymphocytes from CD1 mice that consumed sweeteners for 12 weeks.
| Baseline | Middle (9th week) | Final (15th week) | |||||||
|---|---|---|---|---|---|---|---|---|---|
| CL | SUC | SUCL | ST | CL | SUC | SUCL | ST | ||
| Mean ± SD % (n = 8) |
Mean ± SD % (n = 8) |
Mean ± SD % (n = 8) |
Mean ± SD % (n = 8) |
Mean ± SD % (n = 8) |
Mean ± SD % (n = 8) |
Mean ± SD % (n = 8) |
Mean ± SD % (n = 8) |
Mean ± SD % (n = 8) |
|
| IFN-γ | 15.52 ± 0.106 | 13.71 ± 0.053 | 12.06 ± 0.106 | 17.63 ± 0.106∗ | 11.03 ± 0.053∗ | 9.15 ± 0.053 | 3.73 ± 0.106 | 5.92 ± 0.106 | 5.36 ± 0.053 |
| TNF-α | 5.99 ± 0.106 | 4.47 ± 0.106 | 5.96 ± 0.096 | 7.31 ± 0.08∗ | 8.77 ± 0.096∗ | 4.47 ± 0.106 | 5.73 ± 0.096 | 7.41 ± 0.08 | 8.81 ± 0.096 |
| IL-4 | 8.84 ± 0.08 | 7.54 ± 0.106 | 9.89 ± 0.096 | 18.29 ± 0.058∗ | 4 ± 0.053 | 6.82 ± 0.106 | 6.52 ± 0.096 | 7.6 ± 0.058 | 2.2 ± 0.053 |
| IL-5 | 11.69 ± 0.106 | 13.95 ± 0.08 | 11.36 ± 0.08 | 19.55 ± 0.085∗ | 10.45 ± 0.08∗ | 11.23 ± 0.09 | 3.26 ± 0.08 | 6.45 ± 0.09 | 4.66 ± 0.07 |
| IL-10 | 10.89 ± 0.08 | 8.95 ± 0.133 | 8.96 ± 0.053 | 16.56 ± 0.056∗ | 2.39 ± 0.08∗ | 9.5 ± 0.133 | 7.3 ± 0.053 | 8.72 ± 0.096 | 2.2 ± 0.08 |
The values represent the mean ± SD of percentage of intracellular cytokines (CD3+/CD4+ T cells) determined by flow cytometry from lamina propria of CD1 mice at 3 (baseline), 9 (middle), and 15 (final) weeks of age. One-way ANOVA was performed to compare the differences between the subgroups. The differences were considered significant with a value of p < 0.05∗. CL: Control, SUC: Sucrose, SUCL: Sucralose, ST: Stevia, IFN-γ: interferon-gamma, TNF-α: tumor necrosis factor-alpha, IL-4: interleukin 4, IL-5: interleukin 5, and IL-10: interleukin 10.