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. Author manuscript; available in PMC: 2018 May 9.
Published in final edited form as: Neuron. 2018 Jan 27;97(3):538–554.e5. doi: 10.1016/j.neuron.2018.01.007

Figure 4. NGBs Produce Leg Motor Neurons and All Thoracic NG.

Figure 4

(A and B) Thoracic segments of an L3 VNC labeled with anti-BRP (blue) and anti-Dll (red) containing MARCM clones expressing mCD8::GFP under the control of both VGlut-Gal4 and repo-Gal4. Lin A, Lin ?, and Lin D clones produce motor neurons (arrowhead) and glia (arrow). (A1–A5) Sample with Lin A, Lin B and Lin ? clones; note that Lin B does not produce glia. (B1–B5) Sample with a Lin D clone. Asterisks mark motor neuron axons exiting the VNC.

(C–E) Lin A (C1–C3), Lin ? (D1–D3) and Lin D (E1–E3) cis2 MARCM clones in a ProN labeled with anti-BRP (blue) (C1–C2, D1–D2, E1–E2) and in a T1 Leg (C3, D3, E3) expressing mCD8::RFP in NG (red) and mCD8::GFP (green) under the control of repo-Gal4 and VGlut-QF, respectively.