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. 2018 May 2;9:825. doi: 10.3389/fimmu.2018.00825

Figure 6.

Figure 6

Real-time trafficking of natural killer (NK) cells in a human breast cancer xenograft model. Expanded NK cells were stained with 0.2 µM of ESNF13 and intravenously injected into MDA-MB-231 tumor-bearing NSG mice after 44 days tumor inoculation. (A) The fluorescent signal was detected using a near-infrared (NIR) fluorescent system. After 4 h, the mice were sacrificed and resected organs were explored. (B) The lung, liver, and tumor were resected 4 h after injection of ESNF13-stained NK cells and were stained with anti-HLA or anti-CD56 to detect the tumor and infiltrative CD56+ NK cells. The tumor cells were highlighted with HLA class 1 antibody and the localization of the NK cells corresponding to the scan imaging was demonstrated with CD56 immunohistochemistry. The lung showed a higher population of NK cells at the apex and the tumor specimen displayed an NK cell population mainly at the tumor margin. In accordance with fluorescence signal below detection limit, the liver had only micrometastatic tumors and few CD56+NK cells.