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. 2018 May 2;9:942. doi: 10.3389/fimmu.2018.00942

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Copyright © 2018 Stanisavljević, Dinić, Jevtić, Đedović, Momčilović, Đokić, Golić, Mostarica Stojković and Miljković.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of antibiotic (Antb) treatment on PLN cells (PLNC). Albino Oxford rats were untreated [control (Ctrl)] or treated with Antb and they were immunized with spinal cord homogenate + complete Freund’s adjuvant (CFA) (A–C,G,H) or with myelin basic protein + CFA (D–F). PLNC were isolated from rats at 4, 7, and 14 days post immunization (d.p.i.). Cellularity (A), CD4 proportion (B), regulatory T cell (Treg) proportion (C), interferon (IFN)-γ levels (D), IL-17 levels (E), and IL-10 levels (F) were determined. The total number of samples obtained in at least three independent experiments was 20 (A), 24 (B,C), and 9 (D–F) per group. Data are presented as mean ± SD. Representative plots obtained at 4 d.p.i. are showing gating strategy for Treg (G,H). *p < 0.05 Antb vs. Ctrl (t-test).