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. 2018 May 2;9:942. doi: 10.3389/fimmu.2018.00942

Figure 5.

Figure 5

Effects of Albino Oxford (AO) gut microbiota transfer on experimental autoimmune encephalomyelitis in Dark Agouti (DA) rats. DA rats were untreated [control (Ctrl)] or treated with AO gut microbiota (Gmbt) as represented in scheme (A). They were immunized with spinal cord homogenate + complete Freund’s adjuvant and clinical score was determined daily (B). The total number of samples obtained in two independent experiments was 14 per group. Results are presented as mean ± SE. Spinal cords were isolated at 14 days post immunization (d.p.i.) and cytokines were measured in supernatants obtained by centrifugation of spinal cord homogenates (C). The total number of samples obtained in two independent experiments was four per group. Results are presented as mean ± SD. Popliteal lymph node (PLN) (D–F) and mesenteric lymph node (G–I) were isolated at 4 and 7 d.p.i. Cellularity (D,G), percentage of CD4+ T cells (E,H) and regulatory T cells (Treg) (F,I) were determined. The total number of samples obtained in two independent experiments was five (D,E,G,H), and nine (F,I) per group. Results are presented as mean ± SD. Representative plots obtained at 4 d.p.i. are showing gating strategy for Treg in PLN cells (PLNC) (J) and MLN cells (MLNC) (K). *p < 0.05 Gmbt vs. Ctrl [U test (B), t-test (C–I)].