Table 1.
Primer pairs used in PCR preparing for ISH probes.
| Primer | Sequence (5′->3′) | Product (nt) |
|---|---|---|
| SST | 391 | |
| Forword | CTCTGCATCGTCCTGGCTT | |
| Rerverse | GGGGCCAGGAGTTAAGGAAG | |
| EX+T7+Reverse | CAGTGAATTGTAATACGACTCACTATAGGGAGAGGGGCCAGGAGTTAAGGAAG | |
| NPSR1 | 741 | |
| Forword | CCAGAATGAGAGCTGAGACCTT | |
| Rerverse | TCTGCCTAAAGAGATGCTTTCC | |
| EX+T7+Reverse | CAGTGAATTGTAATACGACTCACTATAGGGAGA TCTGCCTAAAGAGATGCTTTCC | |
| CCKBR | 743 | |
| Forword | GATGGTGATAATGACAGCGAGA | |
| Rerverse | ACTGCTTTCTGTGTGTAGGGGT | |
| EX+T7+Reverse | CAGTGAATTGTAATACGACTCACTATAGGGAGA ACTGCTTTCTGTGTGTAGGGGT |
The first pair of primers contained the forward and reverse primer, used in the first PCR. The second pair of primers containing forward and EX+T7+reverse primer was used in the second PCR. EX, extra nucleotides; T7, T7 RNA polymerase promoter. Genebank accession no.: SST, somatostatin, NM_009215.1 (refer to https://www.ncbi.nlm.nih.gov/nuccore/6678034); NPSR1, neuropeptide S receptor 1, NM_175678.2 (refer to http://mouse.brain-map.org/experiment/show/70560288); CCKBR, cholecysto-kinin B receptor, NM_007627.5 (refer to http://mouse.brain-map.org/experiment/show/70560342).