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. 2018 May 2;9:831. doi: 10.3389/fmicb.2018.00831

FIGURE 4.

FIGURE 4

Multiple alignment of the amino terminal sequences of various NhaD homologs by Clustal_X. Residues in blue squares are the replaced residues for chimera N463r-C7 construction; residues in TM XIII for mutation are shown in red squares. Amino acid sequence of NhaD2 was used as a query sequence for BlastP analysis. Homologous sequences from Alkalimonas amylolytica (Aa-NhaD, No. Q56EB3), Vibrio parahaemolyticus (Vp-NhaD), Vibrio vulnificus (Vv-NhaD, No. OJI52092), Vibrio cholera (Vc-NhaD, No. A5F120), and Shewanella oneidensis (So-NhaD, No. Q8EIA8) were selected for alignments.