Table 1.
Strains used or generated in this study.
| Strain | Relevant phenotype or genotype | Reference |
|---|---|---|
| Halomonas sp. Y2 | Moderate halophilic alkalophilic strain | Yang et al., 2010 |
| E. coli C43 | Competent cell for expression | |
| E. coli DH5α | Competent cell for cloning | |
| E. coli KNabc | ΔnhaA, ΔnhaB, and ΔchaA | Nozaki et al., 1998 |
| NhaD1 chimerical strain | ||
| KNabc/N40r | E. coli KNabc with N40r | This study |
| KNabc/N67r | E. coli KNabc with N67r | This study |
| KNabc/N129r | E. coli KNabc with N129r | This study |
| KNabc/N202r | E. coli KNabc with N202r | This study |
| KNabc/N267r | E. coli KNabc with N267r | This study |
| KNabc/N292r | E. coli KNabc with N292r | This study |
| KNabc/N358r | E. coli KNabc with N358r | This study |
| KNabc/N425r | E. coli KNabc with N425r | This study |
| KNabc/N463r | E. coli KNabc with N463r | This study |
| NhaD2 chimerical strain | ||
| KNabc/N40 | E. coli KNabc with N40 | This study |
| KNabc/N67 | E. coli KNabc with N67 | This study |
| KNabc/N129 | E. coli KNabc with N129 | This study |
| KNabc/N202 | E. coli KNabc with N202 | This study |
| KNabc/N267 | E. coli KNabc with N267 | This study |
| KNabc/N292 | E. coli KNabc with N292 | This study |
| KNabc/N358 | E. coli KNabc with N358 | This study |
| KNabc/N425 | E. coli KNabc with N425 | This study |
| KNabc/N463 | E. coli KNabc with N463 | This study |
| KNabc/N463r-C7 | E. coli KNabc with N463r-C7 | This study |
| N463r-C7 mutant | ||
| KNabc/N463r-C7-V466A | E. coli KNabc with N463r-C7-V466A | This study |
| KNabc/N463r-C7-A468V | E. coli KNabc with N463r-C7-A468V | This study |
| KNabc/N463r-C7-V474I | E. coli KNabc with N463r-C7-V474I | This study |
| KNabc/N463r-C7-A478I | E. coli KNabc with N463r-C7-A478I | This study |
| KNabc/N463r-C7-A479V | E. coli KNabc with N463r-C7-A479V | This study |
| KNabc/N463r-C7-M482W | E. coli KNabc with N463r-C7-M482W | This study |
| KNabc/N463r-C7-I483L | E. coli KNabc with N463r-C7-I483L | This study |
| Halomonas sp. Y2 strain | ||
| Y2/ΔnhaD2 | Y2 with nhaD2 deficiency | Cheng et al., 2016 |
| Y2/NhaD2 | Y2/ΔnhaD2 with NhaD2 | Cheng et al., 2016 |
| Y2/N463r-C7 | Y2/ΔnhaD2 with N463r-C7 | This study |
| Y2/N463r-C7-V466A | Y2/ΔnhaD2 with N463r-C7-V466A | This study |
| Y2/N463r-C7-A468V | Y2/ΔnhaD2 with N463r-C7-A468V | This study |
| Y2/N463r-C7-V474I | Y2/ΔnhaD2 with N463r-C7-V474I | This study |
| Y2/N463r-C7-A478I | Y2/ΔnhaD2 with N463r-C7-A478I | This study |
| Y2/N463r-C7-A479V | Y2/ΔnhaD2 with N463r-C7-A479V | This study |
| Y2/N463r-C7-M482W | Y2/ΔnhaD2 with N463r-C7-M482W | This study |
| Y2/N463r-C7-I483L | Y2/ΔnhaD2 with N463r-C7-I483L | This study |
| E. coli C43 strains FRET analysis | ||
| C43/D2-CFP-YFP | E. coli C43 with D2-CFP-YFP | Meng et al., 2017 |
| C43/N463r-CFP-YFP | E. coli C43 with N463r-CFP-YFP | This study |