Table 3.
Apparent Km values of constructed fusion proteins in TM XIII.
| Variants | Apparent Km (mM) |
|
|---|---|---|
| Na+ | Li+ | |
| NhaD1 | 0.89 ± 0.05 | 2.43 ± 0.12 |
| NhaD2 | 0.47 ± 0.02 | 2.07 ± 0.11 |
| N463r-C7 | 0.33 ± 0.01 | 1.44 ± 0.12 |
| N463r-C7-V466A | 0.49 ± 0.05 | 2.29 ± 0.08 |
| N463r-C7-A468V | – | – |
| N463r-C7-V474I | 0.48 ± 0.02 | 2.95 ± 0.06 |
| N463r-C7-A478I | 0.42 ± 0.03 | 2.82 ± 0.11 |
| N463r-C7-A479V | 0.37 ± 0.02 | 2.64 ± 0.14 |
| N463r-C7-M482W | 1.67 ± 0.06 | 5.00 ± 0.21 |
| N463r-C7-I483L | 0.59 ± 0.05 | 2.48 ± 0.08 |
The everted membrane activities of these proteins were measured in buffer B solution at pH 8.5, with different concentrations of NaCl or LiCl (0.01–100 mM) supplementation. “–” indicates no activity and not determined. The activity of each sample was determined in triplicate.