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Effect of TD-3 on the oxygen affinity of NEM-modified Hb (A) and Hb C93A (B). NEM treatment blocked the ability of TD-3 to further decrease the P50 of HbA. TD-3 did not decrease the P50 of Hb C93A. Each data point represents the mean P50, and error bars represent standard deviation (sd) of the P50 measured at 37 °C in triplicate. Most error bars are too short to visualize because the sd was less than 0.7 mmHg.
