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Purification of the recombinant Arabidopsis SSADH1
| Step | Total Protein | Total Activity | Specific Activity | Yield | Purification |
|---|---|---|---|---|---|
| mg | units | units mg−1 protein | % | fold | |
| Soluble protein | 1230.0 | 195 | 0.16 | 100 | 1.0 |
| Q-Sepharose I | 72.8 | 33 | 0.45 | 17 | 2.9 |
| Size exclusion | 41.1 | 29 | 0.71 | 15 | 4.5 |
| Q-Sepharose II | 11.3 | 16 | 1.39 | 8 | 8.8 |
| Blue Sepharose | 0.6 | 14 | 22.70 | 7 | 143.0 |
