Table 3.
Stimulatory response (LSM ± SEM) from experiments 1 and 2 analyzed by 2 methods: experiment 2 only and the first periods from experiments 1 and 21
| Item | Interactions: Feed × LH content | P-value | |||||
|---|---|---|---|---|---|---|---|
|
|
|
||||||
| AL-H | AL-L | FR-H | FR-L | Feed | LH | Feed × LH | |
| Experiment 2 (no.) | 16 | 15 | 15 | 16 | |||
| Follicles >9 mm (no.) | 18.8 ± 2.1 | 16.6 ± 2.1 | 15.8 ± 2.1 | 16.6 ± 2.1 | 0.34 | 0.68 | 0.38 |
| Ovulation rate – 60 h (%) | 81.4 ± 5.3 | 77.1 ± 5.5 | 74.8 ± 5.5 | 76.9 ± 5.3 | 0.83 | 0.74 | 0.39 |
| CL number (no.) | 18.3 ± 2.8 | 15.5 ± 2.8 | 17.9 ± 2.8 | 14.9 ± 2.8 | 0.78 | 0.10 | 0.96 |
| First period (no.) | 8 | 8 | 8 | 8 | |||
| Follicles >9 mm (no.) | 22.0A ± 3.6 | 17.9AB ± 3.6 | 13.6B ± 3.6 | 19.1AB ± 3.6 | 0.33 | 0.85 | 0.19 |
| Ovulation rate – 60 h (%) | 87.3a ± 6.9 | 63.5b ± 6.9 | 85.5a ± 6.9 | 92.3a ± 6.9 | 0.06 | 0.23 | 0.04 |
| CL number (no.) | 22.1 ± 3.9 | 16.6 ± 3.9 | 17.0 ± 3.9 | 19.4 ± 3.9 | 0.76 | 0.69 | 0.32 |
a,b
Within response variable, means with different superscripts differ (P < 0.05)
A,B
Within response variable, means with different superscripts tended to differ (P < 0.15).
1
Superstimulated, lactating Holstein dairy cows were fed either ad libitum (AL) or feed restricted (FR) and exposed to either low LH (L) or high LH (H) to create 4 treatments: AL-H, AL-L, FR-H, and FR-L.