Table 4.
Uterine flush results (LSM ± SEM) from experiments 1 and 2 analyzed by 2 methods: experiment 2 only and the first periods from experiments 1 and 21
| Item | Interactions: Feed × LH content | P-value | |||||
|---|---|---|---|---|---|---|---|
|
|
|
||||||
| AL-H | AL-L | FR-H | FR-L | Feed | LH | Feed × LH | |
| Experiment 2 (no.) | 16 | 15 | 14 | 16 | |||
| Embryos/oocytes recovered (no.) | 11.6 ± 2.3 | 8.3 ± 2.4 | 9.6 ± 2.4 | 10.1 ± 2.3 | 0.95 | 0.36 | 0.21 |
| Recovery rate (%) | 59.0 ± 8.9 | 53.6 ± 9.6 | 61.7 ± 9.2 | 53.6 ± 8.9 | 0.88 | 0.46 | 0.88 |
| Fertilized oocytes (no.) | 6.2 ± 1.7 | 4.9 ± 1.8 | 4.6 ± 1.8 | 5.0 ± 1.7 | 0.51 | 0.67 | 0.46 |
| Unfertilized oocytes (no.) | 5.4 ± 1.4 | 3.1 ± 1.5 | 4.7 ± 1.5 | 5.1 ± 1.4 | 0.95 | 0.20 | 0.24 |
| Fertilization rate (%) | 42.5 ± 12.1 | 55.8 ± 12.6 | 56.8 ± 12.4 | 47.7 ± 12.7 | 0.76 | 0.79 | 0.19 |
| First period (no.) | 8 | 8 | 7 | 8 | |||
| Embryos/oocytes recovered (no.) | 14.5a ± 2.9 | 9.1ab ± 2.9 | 4.7b ± 3.2 | 13.4a ± 2.9 | 0.36 | 0.59 | 0.03 |
| Recovery rate (%) | 57.8ab ± 10.6 | 55.6ab ± 11.5 | 35.6b ± 11.5 | 72.1a ± 10.6 | 0.80 | 0.13 | 0.09 |
| Fertilized oocytes (no.) | 7.1AB ± 2.3 | 7.8AB ± 2.3 | 3.1B ± 2.5 | 8.1A ± 2.3 | 0.44 | 0.24 | 0.35 |
| Unfertilized oocytes (no.) | 7.4a,A ± 1.5 | 0.8b,B ± 1.5 | 1.6b,AB ± 1.6 | 5.3a,A ± 1.5 | 0.81 | 0.22 | <0.01 |
| Fertilization rate (%) | 47.9b ± 10.0 | 89.4a ± 10.8 | 80.1a ± 10.8 | 59.9b ± 10.0 | 0.90 | 0.32 | <0.01 |
a,b
Within response variable, means with different superscripts differ (P < 0.05)
A,B
Within response variable, means with different superscripts tended to differ (P < 0.15).
1
Superstimulated, lactating Holstein dairy cows were fed either ad libitum (AL) or feed restricted (FR) and exposed to either low LH (L) or high LH (H) to create 4 treatments: AL-H, AL-L, FR-H, and FR-L.