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. 2018 Feb 4;470(5):717–731. doi: 10.1007/s00424-018-2113-4

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — The higher temperature in the nucleus of COS7 cells observed with FPT. a Fluorescence lifetime image of FPT. Scale bar indicates 10 μm. b The temperature distribution in the local area of cell. N represents the location of the nucleus. Scale bar indicates 10 μm. c Higher temperature in the nucleus. Histograms of the fluorescence lifetime in the nucleus and in the cytoplasm in a representative cell (the leftmost cell in a). d Histogram of the temperature difference between the nucleus and the cytoplasm (n = 62 cells). ΔTemperature was calculated by subtracting the average temperature of the cytoplasm from that of the nucleus. <ΔT> represents an average of the histogram. This figure is reproduced from Figures 4 and 6 in [52]