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. Author manuscript; available in PMC: 2018 May 9.
Published in final edited form as: J Am Chem Soc. 2017 Dec 13;139(51):18522–18535. doi: 10.1021/jacs.7b07659

Figure 7.

Figure 7

MAP photoblocks D2R. (A) When MAP is attached to I184C, photoswitching from 360 and 460 nm light decreases both basal and DA-evoked current. (B) A surface representation of a D2R homology model. Fourteen residues surrounding the dopamine binding site of D2R were individually mutated to cysteines. These sites were located on the extracellular face of TM2 (orange), EL1 (blue), EL2 (green), or extracellular face of TM7 (cyan). Protonated dopamine is shown bound to the orthosteric binding site (OBS) of D2R (red). (C) Summary of photoblock of cysteine mutants of D2R by MAP. Error bars represent SEM for n = 3–7 cells per cysteine mutant.