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. 2018 May 9;13(5):e0195912. doi: 10.1371/journal.pone.0195912

Fig 4. TCF increases Atgl levels in SkM.

Fig 4

(A) Heatmap obtained by analysing data from the publicly available Gene Expression Omnibus (GEO) data set (GDS3893) revealed an increase of lipid catabolic genes in SkM of mice subject to fasting versus mice fed with ND. (B) Schematic representation of alternate fasting (AF) and time-controlled fasting (TCF). (C) Western blot analysis of Atgl in total homogenates of GSCN and TA from mice fed with ND or subject to AF for 2 cycles. Immunoblots reported are representative of three mice per group out of five giving similar results. Tubulin was used as loading control. (D) Western blot analysis of Atgl in total homogenates of GSCN and TA from mice fed with ND or subject to TCF for 2 cycles. Immunoblots reported are representative of three mice per group out of five giving similar results. Below are reported the densitometric analyses of the immunoreactive bands normalized to Tubulin. Student’s t-test was used to compare ND vs TCF. (E) Western blot analysis of CV-ATP5A and CIII-UQCRC2 in crude mitochondria isolated from GSCN or TA of mice fed with ND or subject to 2 cycles of TCF. Immunoblots reported are representative of three mice per group out of five giving similar results. Grp75 was used as loading control.