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. 2018 May 9;13(5):e0195912. doi: 10.1371/journal.pone.0195912

Fig 6. TCF limits mitochondrial damage and increases Atgl levels.

Fig 6

(A) Western blot analysis of protein carbonyls in crude mitochondria from different tissues of mice fed with HFD/ND or HFD/TCF. vDAC1 was used as loading control. Immunoblots reported are representative of two mice per group out of five giving similar results. The same vDAC1 was used as loading control in Panel C in S1 Fig. (B) mtDNA damage measured by calculating the ratio of long fragment intensity (LFI) and short fragment intensity (SFI) in different tissues of mice fed with HFD/ND or HFD/TCF. BAT: brown adipose tissue, GSCN: gastrocnemius. (C) Western blot analysis of Atgl in GSCN homogenates of mice fed with HFD/ND or HFD/TCF. Immunoblot reported is representative of three mice per group out of five giving similar results. Ponceau staining was used as loading control. Student’s t-test was used to compare HFD/ND vs HFD/TCF.