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Dependence of Glc uptake rate on the pH of the medium in the presence or absence of the uncoupler CCCP (2 μm). The Glc concentration in the incubation medium was 0.5 mm and incubation times were 5 min. CCCP in ethanol was added 1 min before the addition of [14C]Glc. In both treatments the ethanol concentration was 0.8%. The pH values of the MES-Tris buffers (33 mm) were determined with a pH electrode. Curves are third-order regressions.
