Table II.
Inhibition of the uptake of [14C]Glc or [14C]Suc by CCCP
Concentration of CCCP | Glc Uptake | Suc Uptake |
---|---|---|
μm | % of uninhibited uptake | |
0 | 100 | 100 |
2 | 34 | 58 |
5 | 23 | 41 |
10 | 15 | n.d.a |
Ethanolic CCCP was added 1 min before the addition of [14C]Glc or simultaneous with [14C]Suc, respectively. Incubation time was 5 min (Glc) or 15 min (Suc) at pH 5.5. The concentration of Glc and Suc was 0.5 mm; that of ethanol 0.8% (v/v), also in the controls (n = 3).
n.d., Not determined.