Table 4. Number of sequences, OTUs (97% sequence similarity), and richness, and diversity indexes estimated for the microbiome of each holobiont, holothurian feces, and seawater collected from the aquaculture tank.
Samples | Total number of sequences (reads) |
Detected richness OTUs (S) |
Diversity (Shannon–Wiener, H) |
Species evenness (Pielou’s index, J) |
Alpha diversity (α) |
Estimated Richness (S Chao ± se Chao) |
Estimated Richness (S ACE ± se ACE) |
---|---|---|---|---|---|---|---|
Anemonia sulcata (SamplesM1+M2) |
58839 | 5483 | 4.94 | 0.57 | 1478 | 10887 ± 272 | 11750 ± 71 |
Actinia equina (Samples M7 + M8) |
4065 | 254 | 2.99 | 0.54 | 60 | 630 ± 87 | 821 ± 20 |
Holothuria forskali (Samples M5+M6) |
375177 | 15898 | 4.35 | 0.45 | 3366 | 54867 ± 1240 | 54898 ± 52 |
Holothuria tubulosa (Samples M3+M4) |
392169 | 38713 | 6.60 | 0.62 | 10658 | 63609 ± 475 | 70507 ± 171 |
Holothurian feces (Sample M9) |
10838 | 462 | 2.37 | 0.39 | 98 | 2109 ± 330 | 1896 ± 27 |
Seawater (Sample M10) |
37215 | 2053 | 4.49 | 0.59 | 468 | 4596 ±219 | 4616 ± 43 |