Table 1. Sequences of primers and probes used for real-time PCR.
| Primer or probe | Primer direction | Sequence |
|---|---|---|
| 18s | Forward | CGGCTACCACATCCAAGGAA |
| Reverse | GCTGGAATTACCGCGGCT | |
| Probe | TGGCACCAGACTTGCCCTC | |
| TGFβ | Forward | GCAACATGTGGAACTCTACCAGAA |
| Reverse | GACGTCAAAAGACAGCCACTCA | |
| TNFα | Forward | CTGTCTACTGAACTTCGGGGTGAT |
| Reverse | CGGA(C/T)GTAAGGGCCGTGC | |
| IL-1β | Forward | GGCCTCAAAGGAAAGAATCTATACC |
| Reverse | GCTCTGGGCCATAGAACTGATG | |
| Probe | ATGAGAAGTTCCCAAATGGCCTCCCTC | |
| iNOS | Forward | CAGCTGGGCTGTACAAACCTT |
| Reverse | GTATTGCTTGGGATCCACACTCT | |
| Probe | ATGAAAGACGGCACACCCACCCTG | |
| GPR41 | Forward | GTGACCATGGGGACAAGCTTC |
| Reverse | CCCTGGCTGTAGGTTGCATT | |
| GPR43 | Forward | GGCTTCTACAGCAGCATCTA |
| Reverse | AAGCACACCAGGAAATTAAG | |
| GPR109A | Forward | GGCGTGGTGCAGTGAGCA |
| Reverse | CTGCAGGACTCTGTCTTCTCAGT |
Primers and probes were purchased from (Thermo Fisher, Waltham, MA).