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. 2018 May 9;13(5):e0196973. doi: 10.1371/journal.pone.0196973

Table 1. The emerging time of embryonic structures in Taiwan Country Chicken (TCC) and Brown Tsaiya Ducks (BTD) during the first 72 h of incubation in comparison with the HH* staging system.

Hour post-incubation
Embryonic structures HH* TCC BTD
Two-layered blastoderm (HH1; D13) ** 0–5 0–5 1–12
Primitive streak (HH2; D14) 6–7 6–7 10–13
Intermediate primitive streak (HH3; D15) 12–13 12–13 19–24
Full-length primitive streak (HH4; D16) 18–19 18–19 25–27
Regressing primitive streak N/A 23–39 28–42
Headfold formation (HH6; D19) 23–25 22–25 31–36
First somite pair (HH7; D20) 23–26 24–26 32–34
Neural fold (HH7; D21) 23–26 24–26 32–34
Primary optic vesicles (HH9; D22) 29–33 27–32 36–42
Paired heart primordia (HH9; D22) 29–33 26–32 36–42
Nervous system
        Three primary brains (HH10; D23) 33–38 33–39 43–46
        Five neuromeres (HH11; D24) 40–45 40–45 46–52
Limb primordia; wing and leg buds (HH17; D30) 51–64 51–64 64–72
Allantois (HH19; D32) 65–72 68–72 N/A

*Hamburger-Hamilton staging system

** The embryonic structures refer to the stages from Hamburger and Hamilton (1915) and Dupuy et al. (2002), designated as D-staging or D.

N/A: data not available.