Fig 10. Multiplicity of infection exaggerates size and quantity of WT PV-induced vesicular clusters.

Transmission electron micrographs (TEM) of HeLa cells infected with WT PV at an MOI of 10 (A), 50 (B) or 100 (C). Vesicular clusters are indicated by white dotted circles. Note that the size of the vesicular clusters increases with increasing MOI. HeLa cells were infected with WT PV expressing EGFP at an MOI of 10, 50 or 100 by incubating the monolayer with WT virus for 30 min at room temperature, followed by removal of the virus and washing of the monolayer with PBS. Prewarmed media was then added to the monolayer and incubated at 37°C. Samples were fixed 3 h post-infection and visualized by TEM. Bar = 1 μm. N denotes nucleus.