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. 2018 Apr 27;14(4):e1007295. doi: 10.1371/journal.pgen.1007295

Fig 6. set-17 promotes expression of msp genes in spermatogenic gene clusters.

Fig 6

A) Expression categories defined by Ortiz et al. (2014) of 123 transcripts misregulated in set-17 mutants based on RNAseq studies of whole L4 hermaphrodites. Sperm, enriched in sperm-producing germlines (60, red); sperm + oocyte, genes expressed similarly in sperm and oocytes (40, dark grey); non-germline, genes not detected in dissected germlines (23, light grey). We did not detect genes enriched in oocytes but not sperm. B) set-17 promotes expression of a subset of spermatogenic transcripts. Average log2-fold change in expression vs. the wild-type for all 123 set-17 misregulated transcripts (set-17 all) in set-17 mutants; for the 60 set-17 misregulated spermatogenic transcripts (set-17 sperm.) in set-17 mutants, in set-17; Pset-17::set-17(+) and in set-17; Pmex-5::set-17(+) mutants, respectively; and for all 2,306 previously identified spermatogenic transcripts (All sperm.) in set-17 mutants. **** P < 0.0001, MW exact or KS test. C) set-17 promotes msp expression in the germline. log2-fold change in expression vs. the wild type for all 28 msp gene transcripts (msp genes) in set-17, set-17; Pset-17::set-17(+) and set-17; Pmex-5::set-17(+) mutants. **** P < 0.0001, MW exact or KS test. D), E) set-17 spermatogenic genes cluster in the genome. Positional distributions of spermatogenic genes and msp genes on chromosomes II and IV and spermatogenic set-17-misregulated genes on chromosomes II and IV. Above: histograms of spermatogenic genes on chromosomes (D) II and (E) IV. Blue, spermatogenic gene clusters. Below: log2-fold change in expression in set-17 vs. the wild type from RNAseq studies in whole L4 hermaphrodites for the set-17 spermatogenic genes (red) on chromosomes (D) II and (E) IV. Yellow, msp genes. F) set-17 promotes expression of spermatogenic genes in a cluster on chromosome II. log2-fold change in expression vs. wild type from RNAseq studies of whole L4 hermaphrodites for all spermatogenic genes in the spermatogenic gene cluster on chromosome II (blue region in 5D) in set-17 (blue), set-17; Pset-17::set-17(+) (orange) and set-17; Pmex-5::set-17(+) (yellow) as well as for all spermatogenic genes outside of the blue region on chromosome II (grey). Mean +\-95% CI; **** P < 0.0001, MW exact or KS test. G) set-17 promotes expression of spermatogenic genes in clusters on chromosome IV. log2-fold change in expression vs. the wild type from RNAseq studies of whole L4 hermaphrodites for all spermatogenic genes in the spermatogenic gene clusters on chromosome IV (blue regions in 5E) in set-17 (blue), set-17; Pset-17::set-17(+) (orange) and set-17; Pmex-5::set-17(+) (yellow), as well as for all spermatogenic genes outside of the blue region on chromosome IV (grey). Mean +\-95% CI; **** P < 0.0001, MW exact or KS test. H) set-17 promotes expression of spermatogenic genes in a cluster on chromosome V. log2-fold change in expression vs. the wild type from RNAseq studies of whole L4 hermaphrodites for all spermatogenic genes in the newly identified spermatogenic gene cluster on chromosome V (blue region in S5K) in set-17 (pink), set-17; Pset-17::set-17(+) (orange) and set-17; Pmex-5::set-17(+) (yellow) as well as for all spermatogenic genes outside of the blue region on chromosome V (grey). Mean +\-95% CI shown. Cl. V (set-17(-)) vs. Non-Cl. V (set-17(-)), ** P < 0.005, MW exact; Cl. V (set-17(-)) vs. Cl. V (Pset-17::set-17(+)), *** P < 0.0005, Wilcoxon test; Cl. V (set-17(-)) vs. Cl. V (Pmex-5::set-17(+)), **** P < 0.0001, Wilcoxon test.