Fig 3. Late σF-dependent expression of both PsigG and PspoIIQ requires SpoIIQ, σE, and SpoIIIAA-AH.
The activity of PsigG-lacZ (A, C, D) or PspoIIQ-lacZ (B, E) was monitored during sporulation of strains with the following genotypes: wild type (WT; open circles), ΔsigG (closed circles), ΔsigG ΔsigF (open squares), ΔsigG ΔspoIIQ (open diamonds), ΔsigG ΔsigE (open triangles), and ΔsigG ΔspoIIIAA-AH (closed triangles). (PsigG-lacZ strains were JJB31, JJB73, JJB75, JJB79, JJB85, and JJB77, respectively. PspoIIQ-lacZ strains were AHB881, AHB882, AHB915, AHB916, AHB917, and AHB1017, respectively.) For clarity, only data from a subset of these strains are presented in each graph (as labeled) and, also for clarity, the data for the ΔsigG strain of each (closed circles) is presented in all graphs. Note that (D) provides a zoomed view of the data from the boxed area of (C). The timing of the σF-to-σG switch, between sporulation hours 2.5 and 3, is indicated in each panel by a dashed gray line. For all panels, error bars indicate ± standard deviations based on three independent experiments.