Table I.
Summary of the experimental conditions (day/night temperatures and length of treatment) for growing winter rye seedlings and applying cold and snow mold treatments
| Samplea | Growth | Cold Treatment | Snow Mold Treatment | Age at Harvest |
|---|---|---|---|---|
| NA20 | 20°C/16°C, 3 weeks | – | – | 3 weeks |
| CA5 | 20°C/16°C, 1 week | 5°C/2°C, 7 weeks | – | 8 weeks |
| NA18 | 18°C/18°C, 5 weeks | – | – | 5 weeks |
| NA18 INF | 18°C/18°C, 2–3 weeks | – | 18°C/18°C, 2–3 weeks | 5 weeks |
| NA2 | 18°C/18°C, 2–3 weeks | 2°C/2°C, 4–5 weeks | – | 7–8 weeks |
| NA2 INF | 18°C/18°C, 2–3 weeks | – | 2°C/2°C, 4–5 weeks | 7–8 weeks |
| CA2 | 20°C/16°C, 1 week | 5°C/2°C, 5 weeks + 2°C/2°C, 4–5 weeks | – | 10–11 weeks |
| CA2 INF | 20°C/16°C, 1 week | 5°C/2°C, 5 weeks | 2°C/2°C, 4–5 weeks | 10–11 weeks |
Plants were grown for the indicated times under non-acclimating and cold-acclimating conditions, and half of the plants were inoculated and incubated with snow mold. NA20, NA18, NA2, and CA2 represent control plants for CA5, NA18 INF, NA2 INF, and CA2 INF plants, respectively. The leaves were harvested for apoplastic extractions from at least three (n = 3) independent experiments.
a
CA, cold-acclimated; INF, infected with snow mold; NA, non-acclimated.