Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | Histone Deacetylase 7 (HDAC7) | NA | HDAC7 | Coding sequence used for HDAC7 expression constructs and the HDAC7-ΔP transgene. |
| Gene (Homo sapiens) | Promyelocytic Leukemia, Zinc Finger (PLZF) | NA | ZBTB16 | Coding sequence used for PLZF expression constructs. |
| Strain, strain background (Mus musculus) | C57/BL6 | Jackson Laboratories | Stock Number: 000664 | |
| Strain, strain background (Mus musculus) | Boyj (B6.SJL-Ptprca Pepcb/BoyJ) | Jackson Laboratories; PMID: 11698303 | Stock Number: 002014 | B6 strain congenic for Cd45.1 |
| Genetic reagent (Mus musculus) | Vα14/Jα18 Transgenic, Tg(Cd4-TcraDN32D3)1Aben | Jackson Laboratories; PMID: 18031695 | MGI:4880641 | Vα14/Jα18 Transgenic from Bendelac laboratory |
| Genetic reagent (Mus musculus) | Lck-Cre Transgenic, Tg(Lck-cre)548Jxm | Jackson Laboratories; PMID: 8618846 | MGI: 2176199 | Cre strain for thymicHdac7 deletion |
| Genetic reagent (Mus musculus) | Hdac7flox/flox, Hdac7tm2Eno | Eric Olson, UTSW; PMID: 16873063 | MGI: 1891835 | HDAC7 floxed allele |
| Genetic reagent (Mus musculus) | Hdac7-/+, Hdac7tm1Eno | Eric Olson, UTSW; PMID: 16873063 | MGI: 1891835 | HDAC7 null allele |
| Genetic reagent (Mus musculus) | Lck-PLZF transgenic, C57BL/6-Tg(Cd4-Zbtb16)1797Aben/J | Jackson Laboratories; PMID: 18703361 | MGI:4881493 | PLZF Transgenic strain from Bendelac Laboratory |
| Genetic reagent (Mus musculus) | HDAC7-ΔP Transgenic | Our laboratory; PMID: 23103766 | NA | Transgenic expression of HDAC7-ΔP under control of Lcl proximal promoter/CD2 LCR in C57BL/6 |
| Recombinant DNA reagent | pCDNA 3.1(+) FLAG-HDAC7 FL | this paper | NA | Expression construct for FL FLAG-tagged human HDAC7 |
| Recombinant DNA reagent | pCDNA 3.1(+) FLAG-HDAC7 1–497 | this paper | NA | Expression construct for FLAG-tagged human HDAC7 truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) FLAG-HDAC7 65–497 | this paper | NA | Expression construct for FLAG-tagged human HDAC7 truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) FLAG-HDAC7 120–497 | this paper | NA | Expression construct for FLAG-tagged human HDAC7 truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) FLAG-HDAC7 1–220 | this paper | NA | Expression construct for FLAG-tagged human HDAC7 truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) FLAG-HDAC7 1–180 | this paper | NA | Expression construct for FLAG-tagged human HDAC7 truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) HA-PLZF FL | this paper | NA | Expression construct for FL HA-tagged human PLZF |
| Recombinant DNA reagent | pCDNA 3.1(+) HA-PLZF 1–318 | this paper | NA | Expression construct for HA-tagged human PLZF truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) HA-PLZF 1–405 | this paper | NA | Expression construct for HA-tagged human PLZF truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) HA-PLZF 105–460 | this paper | NA | Expression construct for HA-tagged human PLZF truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) HA-PLZF 280–673 | this paper | NA | Expression construct for HA-tagged human PLZF truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) HA-PLZF 320–673 | this paper | NA | Expression construct for HA-tagged human PLZF truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) HA-PLZF 395–673 | this paper | NA | Expression construct for HA-tagged human PLZF truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) HA-PLZF 455–460 | this paper | NA | Expression construct for HA-tagged human PLZF truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) GAL4-PLZF FL | this paper | NA | Expression construct for GAL4 DNA-binding domain (1-142) fused to full-length PLZF |
| Recombinant DNA reagent | pCDNA 3.1(+) GAL4-PLZF 455–460 | this paper | NA | Expression construct for GAL4 DNA-binding domain (1-142) fused to PLZF1 - 460 truncation mutant |
| Recombinant DNA reagent | pCDNA 3.1(+) GAL4-PLZF 455–460 | this paper | NA | Expression construct for GAL4 DNA-binding domain (1-142) fused to PLZF1 - 318 truncation mutant |
| Software, algorithm | Ingenuity Pathway Analysis | Qiagen | RRID:SCR_008653 | Tool for pathway mapping and other gene ontology analysis from RNAseq transcript abundance data. |
| Software, algorithm | Bowtie 2.0 | Johns Hopkins University; PMID: 22388286 | RRID:SCR_005476 | Tool for aligning raw sequence reads to genome |
| Software, algorithm | SeqMonk | Babraham Institute | RRID:SCR_001913 | Tool for calculating RNA transcript abundances from Bowtie-mapped sequence reads (.SAM files) |