Figure 1.

Mechanical properties and lamina protein levels for five, mechanically distinct cell types. (a) Cellular elastic and viscoelastic properties for NHF, MG-63, KGN, HEK-293T, and SH-SY5Y cells. Cellular elasticity was determined from elastic modulus (E_elastic); cellular viscoelasticity was determined by relaxed modulus (E_R) and apparent viscosity (µ_app). (b and c) Lamin A, C, and B1 representative protein expression. Densitometry values normalized to β-tubulin expression. (d) LMNA and LMNB1 gene expression associated with “stiff” MG-63 and “soft” HEK-293T cells. Mechanical property and gene expression data represented as arithmetic mean ± SD. Statistical significance (p < 0.05) was determined using Kruskal–Wallis non-parametric tests with Tukey post hoc tests. Groups with different letters exhibit statistically significant differences.