Fig. 3. X47 Δnfu mutants show growth deficiency.

H. pylori X47 (WT) cells and isogenic X47 Δnfu mutant cells were grown on BA agar under microaerophilic conditions, resuspended in BHI-βc and used to inoculate 10 mL of BHI-βc (starting OD₆₀₀=0.05, which corresponds to approximately 5 Klett units). Cells were grown for 24 h at 37°C, 200 rpm shaking, in sealed bottles (with side arms) with 170 mL head space filled with 10% H₂, 5% CO₂ and either 5% or 15% O₂ concentration, as indicated (N₂ as balance). A Klett spectrophotometer was used to measure OD at various time points. Results are expressed as (mean and standard deviation) Klett units and represent data obtained from three bottles for each condition.