Table 1.
H. pylori proteins predicted to contain Iron-Sulfur clusters.
| HP number* |
Protein ID |
Proposed function/Name | Putative [Fe-S] cluster # |
Putative ligands# |
|---|---|---|---|---|
| HP0117 | HP0117 | Hypothetical protein | [4Fe-4S] (SAM) | C33 C37 C40 |
| HP0132 | SdaA | L-serine dehydratase | [4Fe-4S] | C341 C383 C394 |
| HP0138 | HP0138 | Hypothetical, iron-sulfur cluster binding protein | 2[4Fe-4S] (ferredoxin) | C314 C318 C321 C325; C364 C368 C371 C375 |
| HP0142 | MutY | A/G-specific adenine glycosylase | [4Fe-4S] | C190 C196 C199 C205 |
| HP0191 | FrdB | Fumarate reductase, iron-sulfur subunit | [2Fe-2S], [3Fe-4S], [4Fe-4S] | C60 C65 C68 C80; C154 C157 C160 C221; C164 C211 C217 |
| HP0207 | ApbC, Mrp | Putative Fe-S carrier, ATPase | [4Fe-4S] | C274 C277 C366 |
| HP0221 | NifU | Scaffold protein NifU | [2Fe-2S], [4Fe-4S] | C161 C163 C196 C199; C54 C82 C131 |
| HP0269 | MiaB-like | tRNA-methylthiotransferase | 2[4Fe-4S] (SAM) | C10 C46 C78; C148 C152 C155 |
| HP0277 | HP0277 | Ferredoxin | 2[4Fe-4S] (ferredoxin) | C9 C13 C16 C20; C38 C41 C51 C55 |
| HP0285 | MiaB | tRNA-methylthiotransferase | 2[4Fe-4S] (SAM) | C11 C45 C74; C144 C148 C151 |
| HP0400 | IspH | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase | [4Fe-4S] | C12 C92 C186 |
| HP0468 | HP0468 | Hypothetical protein | [2Fe-2S] | C235 C271 C307 C418 |
| HP0568 | HP0568 | Hypothetical, iron-sulfur cluster binding protein | [4Fe-4S] SPASM | Unknown |
| HP0585 | Nth | Endonuclease III | [4Fe-4S] | C194 C201 C205 C210 |
| HP0588 | OorD | 2-oxoglutarate oxidoreductase, δ subunit | 2[4Fe-4S] (ferredoxin) | C19 C22 C25 C29; C56 C59 C62 C66 |
| HP0625 | IspG | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase | [4Fe-4S] | C264 C267 C299 E306 |
| HP0631 | HydA | Hydrogenase small subunit | 2[4Fe-4S], [3Fe-4S] | C86 C89 C185 C218; H256 C259 C284 C290; C299 C318 C321 |
| HP0654 | MqnE | Aminodeoxyfutalosine synthase | [4Fe-4S] (SAM) | C59 C63 C66 |
| HP0656 | MqnC | Dehypoxanthinylfutalosine cyclase | [4Fe-4S] (SAM) | C81 C85 C88 |
| HP0665 | HemN | O2-independent coproporphyrinogen-III oxidase | [4Fe-4S] (SAM) | C62 C66 C69 |
| HP0666 | GlpC | anaerobic Glycerol-3-phosphate dehydrogenase | 2[4Fe-4S] (ferredoxin) | C17 C20 C23 C27; C68 C71 C74 C78 |
| HP0734 | RimO | Ribosomal protein S12 methylthiotransferase | 2 [4Fe-4S] (SAM) | C16 C50 C82; C151 C155 C158 |
| HP0768 | MoaA | Molybdenum cofactor biosynthesis protein A | 2[4Fe-4S] (SAM) | C21 C25 C28; C249 C252 C266 |
| HP0779 | AcnB | Aconitase B subunit | [4Fe-4S] | C709 C767 C770 |
| HP0934 | QueE | 7-carboxy-7deazaguanine synthase | [4Fe-4S] (SAM) | C31 C35 C40 |
| HP1089 | AddB | ATP-dependent helicase-nuclease | [4Fe-4S] | C778 C781 C787 |
| HP1109 | PorD | Pyruvate-ferredoxin oxidoreductase, d subunit | 2[4Fe-4S] (ferredoxin) | C58 C61 C64 C68; C88 C91 C94 C98 |
| HP1222 | Dld | D-lactate dehydrogenase | 2[4Fe-4S] (ferredoxin) | C548 C551 C554 C558; C610 C613 C616 C620 |
| HP1226 | HemN | O2-independent coproporphyrinogen-III oxidase | [4Fe-4S] (SAM) | C17 C21 C24 |
| HP1261 | NuoB | NADH-Quinone oxidoreductase, subunit B | [4Fe-4S] | C32 C33 C97 C126 |
| HP1266 | Nqo3 | NADH-Quinone oxidoreductase, NQO3 subunit | 2[4Fe-4S], [2Fe-2S] | C34 C39 C45 C48; C143 C146 C149 C153; C203 C206 C209 C213 |
| HP1268 | Nqo9 | NADH-Quinone oxidoreductase, subunit I | 2 [4Fe-4S] (ferredoxin) | C82 C85 C88 C92; C121 C124 C127 C131 |
| HP1356 | NadA | Quinolinate synthase | [4Fe-4S] | C107 C193 C198 |
| HP1406 | BioB | Biotin synthase | [4Fe-4S](SAM), [2Fe-2S] | C17 C21 C24; C61 C96 C154 R221 |
| HP1428 | RlmN | Dual-specificity RNA methyltransferase | [4Fe-4S] (SAM) | C123 C127 C130 |
| HP1492 | Nfu | Nfu-like protein | [2Fe-2S], [4Fe-4S] | C49 C52 C79 |
| HP1508 | HP1508 | Ferredoxin | 2[4Fe-4S] (ferredoxin) | C249 C252 C255 C259; C273 C276 C279 C283 |
| HP1540 | FbcF | Ubiquinol cytochrome c oxidoreductase | [2Fe-2S] | C98 C104 C116 C118 |
*
HP number refers to strain 26695 (Tomb et al., 1997)
#
based on previously published studies on H. pylori proteins or sequence alignments with homologous proteins.
Abreviations: SAM, S-Adenosyl Methionine ligand; SPASM domain: a domain found in [4Fe-4S] binding maturases of Subtilosin, PQQ, Anaerobic Sulfatases, and Mycofactocin (Haft and Basu, 2011).