Table 1. Peptide Identification Metrics.
Summary of peptide-spectrum-match (PSM), unique peptide, protein, protein group (proteins collapsed into indistinguishable protein identifications), and phosphopeptide identifications in each group of experiments.
Experiment | Trypsin | PSMs (%FDR) | Unique Peptides (% FDR) |
Unique Proteins/Groups (% FDR)* |
Unique Phosphopeptides (% total) |
---|---|---|---|---|---|
AP | − | 68114 (0.1) | 7361 (0.9) | 680/530 (0.9) | 264 (3.6) |
AP | + | 47154 (0.1) | 5224 (0.9) | 905/715 (0.9) | 226 (4.3) |
IMAC | − | 404 (0.2) | 191 (0.5) | 27/18 (0.0) | 107 (56.0) |
IMAC | + | 1554 (0.4) | 514 (0.9) | 21/19 (0.0) | 340 (66.1) |
FDR was calculated at the protein-level