Figure 1. CD4 T cells are predominant in skin after cutaneous candida infection.

The images of dorsal skin (A) of wild-type B6 mice infected with C. albicans. (B) Yeast forms of GFP-expressing C. albicans outside of epidermis at day 0 (top) and hyphae forms of GFP-expressing C. albicans in the dermis at day 3 after candida infection (bottom, blue, dermis). Scale bars, 30 µm. Confocal microscopy of infected ear skin at (C) day 10 (effector) and (D) day 30 (memory) after cutaneous candida infection. MHCII⁺ APCs were green, and CD4⁺ T cells were orange. Scale bars, 100 µm. Arrows indicate CD4⁺ T cells in contact with MHCII⁺ APCs. Data are representative of three experiments. (E) Expression of CD4 and CD8 T cells (gated on CD3⁺CD4⁺ and CD3⁺CD8⁺ cells) and ratios of CD4/CD8 T cells from normal skin, infected dorsal skin (day 7 and day 30). (F) Expression of γδ and αβ T cells (gated on CD3+γ δ+ and CD3+TCRβ+ cells) from normal skin and infected dorsal skin (day 30). (G) The number of γδ and αβ T cells (gated on CD3+γ δ+ and CD3+TCRβ+ cells) from normal skin and infected dorsal skin (day 1, 2, 7, 30). Data are representative of more than five experiments. *** p < 0.001; ns, not significant.