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. 2018 May 9;8:7322. doi: 10.1038/s41598-018-25062-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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H7N9 viral RNA loads in organs obtained from infected birds. Horizontal bars indicate mean viral RNA loads when >1 bird was sampled from a given group at a given time/time window. Broken horizontal is the cut-off threshold, as explained in Fig. 2. (a) Log₁₀ REU values in organs of D0 and R1 chickens (ck) plus R2 turkeys (ty) (8log₁₀EID₅₀ direct infection of D0 chickens); (b) Log₁₀ REU values in organs of D0, R1 and R2 turkeys (8log₁₀EID₅₀ direct infection of D0 turkeys); (c) Log₁₀ REU values in organs of D0 and R2 turkeys (6log₁₀EID₅₀ direct infection of D0 turkeys). Pancreas and kidney specimens were first collected for RNA extraction only when mortality became apparent in R2 turkeys, with the brain specimens collected only at R2 cull (11 dpc).