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. 2018 May 9;8:7322. doi: 10.1038/s41598-018-25062-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changing proportions of polymorphisms at residue 235 in the viral HA gene of directly-inoculated (D0) birds swabbed at 1–4 dpi. (a and b) presents D0 turkeys inoculated with 8log₁₀ and 6log₁₀ EID₅₀ of H7N9 wt respectively, while (c) presents D0 chickens inoculated with 8log₁₀ EID₅₀ of H7N9 wt. The total numbers of swabs of both types investigated at each daily time point are denoted by “n”, with the numbers of buccal and cloacal swabs distinguished in parenthesis (buccal, cloacal). All cloacal swabs contained Q235 except one in panel (b) at 4 dpi which contained L235.