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. 2018 May 9;8:7312. doi: 10.1038/s41598-018-25786-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Diameter of the penis after application of the NE with NO under anesthesia. The diameter of penis after application of the NE with NO increased significantly comparing with the control group treated with the oil without NO (D₀: Diameter of penis without application of oil). (b) Redness value of the penis after application of the NE with NO under anesthesia. The redness value after application of the NE with NO was significantly higher than the control group (R₀: Redness value of penis without application of oil). (c) Blood NOx concentration after application of the NE with NO. The level of blood NOx concentration increased 3-fold after the application of the NE with NO compared with control group.