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. 2018 May 9;8:7353. doi: 10.1038/s41598-018-25585-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic relationships of the genus Brassica inferred from complete chloroplast (A) and 45SnrDNA (B) sequences of 28 Brassica and Raphanus genotypes. Tree was developed using MEGA7 with 1,000 bootstrap replications. The bootstrap values for clades are shown in corresponding branches of the tree. Taxon markers with single and double circles represent diploid and tetraploid genomes, respectively. The circled legend for 45SnrDNA and chloroplast corresponds to each species. Dotted line connects the corresponding allotetradiploid genomes of chloroplast and 45SnrDNA. Species divergence times were inferred from Bayesian analysis, and are shown at the side of the node in million years (my). A. thaliana was used as an outgroup.