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. 2018 May 9;8:7424. doi: 10.1038/s41598-018-25454-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pol I Response to CX-5461 Initiation Inhibition. (a) Bright-field, conventional and super-resolution reconstruction images of the Dendra2-RPA194 U2OS cell line after 48 hours of treatment with CX-5461. Sparse foci appear against a stronger background like in the untreated, interphase nucleus. Many dimmer foci that were not visible in the conventional image appear in the super-resolution reconstruction. (b) A sample time trace of a transient cluster (yellow circle in panel a) in the CX-5461 treated nucleus. The burst of detections occurs after a delay (~15 s, red arrow) after the beginning of acquisition, then abruptly stops, signatures of a transiently lived cluster (Supplementary Fig S3). (c) The fraction of stable vs. transient clusters in control and CX-5461 treated cells. (d) Histogram of transient cluster lifetimes in the CX-5461 treated cells. The mean lifetime is 8.7 ± 0.7 s. (e) Survival curve of stable clusters sizes for control and CX-5461 treated clusters. We analyzed a total of 10 cells imaged on three separate days. The cluster size is measured in counts, i.e., the number of localizations within the cluster.