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. 2018 May 9;8:7420. doi: 10.1038/s41598-018-23884-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cells performing OXPHOS upregulated NRF2 expression. (A) Different hematopoietic cells were incubated in OXPHOS medium or treated with 5 mM DCA for 2 weeks. NRF2 mRNA was quantified by qPCR and values normalized to β-actin mRNA. Results were represented as the % of mRNA compared to cells growing only in glucose medium. Bars show average ± SD of 3 independent experiments performed in triplicate. (B) NRF2 protein expression was analyzed in several cell lines by western blotting (upper panel) or by flow cytometry in OCI-AML3 cells. (C) Hepatocytes from 4 donors were treated with the indicated concentration of DCA for 24 h and ERK5, NRF2, NQO-1 and HO-1 mRNA were analyzed. Bars show average ± SD of the four donors performed in duplicate.