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. 2018 May 9;8:7320. doi: 10.1038/s41598-018-25434-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The timing of RNAi silencing in Sclerotinia sclerotiorum in vitro. Transcript levels were measured at time points 0, 24, 48, 72, and 96 hours post treatment of 500 ng/mL of dsRNA targeting SS1G_01703 (a), SS1G_05899 (b), SS1G_06487 (c), or GFP in liquid culture. Data are relative to GFP-dsRNA control and normalized to reference SsSac7 (SS1G_12350). Data represents 3 biological replicates with error bars representing standard error. To test effect of timing, a one-way ANOVA (with significance of p < 0.05) was performed and followed by a Tukey post hoc test to compare means, where significant differences (p_tukey < 0.05) are denoted with differing letters.