Table 2.
Nine microsatellite loci for the Round Goby, Neogobius melanostomus (following Dufour et al. 2007) with primer sequences, annealing temperature (Ta), and MgCl2 concentration
| Locus | Primer sequence | Ta (°C) | MgCl2 (mM) |
|---|---|---|---|
| Nme1 | F: CGAGCGCTAAAATAGAAGAAAA | 48 | 2.4 |
| R: TCCAGTGGCTTGAGTGATGT | |||
| Nme2 | F: TGTGTAATGACGTGGAATAGCC | 55 | 2.1 |
| R: CAATAGGCCAGGATGAATGAG | |||
| Nme3 | F: GCGGGAGTCAAGAATTGAAC | 48 | 2.4 |
| R: TTGTTAGAATGTATTATGCCATAGCC | |||
| Nme4 | F: TGTGCTTGGTTAAGGTGGTG | 55 | 2.4 |
| R: CCGGACAGAAACAACTTAAAGC | |||
| Nme5 | F: GTCACACCGATCTTCGACTG | 48 | 2.4 |
| R: GATTTACTTGATTCATCACT | |||
| Nme6 | F: GCAACTCAACCTCGTAGCC | 59 | 1.9 |
| R: CCGAAAAGCCAATTAAGCAC | |||
| Nme7 | F: AATGGATGGGTCAATTGCAT | 48 | 2.4 |
| R: AAGGTTGAGCTGCCACTGAG | |||
| Nme9 | F: GGGGTGCACTTGTTTAGCTC | 59 | 2.4 |
| R: AACGGACAAGTGGAAGAAGG | |||
| Nme10 | F: GCGATTATGAGGTTCGGAGA | 48 | 2.4 |
| R: ATCAGCAACCCCTGAACAGA |