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. 2018 May 9;9:1842. doi: 10.1038/s41467-018-04239-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Sema7A promotes dendrite maturation and synapse formation. Left, Sema7A levels in the MOR29B and MOR29A glomeruli. OB sections at P4 were immunostained with anti-Sema7A antibodies, and signal intensities of Sema7A are compared (see also Fig. 1c). ^*p < 0.05 (Student’s t-test). Error bars indicate standard deviation SD (n = 8 glomeruli). Middle, Pre- and post-synaptic markers in the MOR29A and MOR29B glomeruli. OB sections at P4 were also immunostained with antibodies against vGlut2 and GluR1, pre- and post-synaptic markers, respectively. Relative fluorescent signals (MOR29B/MOR29A glomeruli) are compared. ^*p < 0.05 (Student’s t-test). Error bars indicate standard deviation SD (n = 8 glomeruli). Right, Dendrite maturation of M/T cells in the MOR29A and MOR29B glomeruli. M/T-cell dendrites were visualized by LY injection into the MOR29A and MOR29B glomeruli at P4. The ratios (%) of mature and immature M/T cells in the MOR29A and MOR29B glomeruli are shown: MOR29A, 8/29 (27.6 %); MOR29B, 22/28 (78.6%). n = 16 glomeruli