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. 2018 May 9;9(5):522. doi: 10.1038/s41419-018-0611-0

Fig. 2. PTP1B functions as an oncogene to promote migration and inhibit apoptosis of GC cells.

Fig. 2

a–c The efficiency of PTP1B overexpression and knockdown in GC cells. a qRT-PCR analysis of PTP1B mRNA levels in MKN45 and MGC803 cells treated with PTP1B plasmid, control plasmid, PTP1B siRNA, or scrambled control siRNA. b,c Western blottings of PTP1B protein in MKN45 and MGC803 cells treated with PTP1B plasmid, control plasmid, PTP1B siRNA, or scrambled control siRNA. b Representative images; c quantitative analysis. d,e Transwell assays were performed after the transfection of MKN45 and MGC803 with PTP1B plasmid, control plasmid, PTP1B siRNA, and control siRNA. d Representative images; Scale bar, 100 μm. e Quantitative analysis. f,g The apoptosis assays were performed 24 h after the transfection of MKN45 and MGC803 cells with PTP1B plasmid, control plasmid, PTP1B siRNA, and control siRNA. f Representative images; g quantitative analysis. n = 3 independent repeats. Data are represented as mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001; two-tailed Student’s t-test