Fig. 3. Decreased expression of GADD45α contributes to development of radioresistance in cervical cancer.

a Transfection of GADD45α shRNA significantly decreased GADD45α expression compared to vector control in HeLa cells. After 72 h of GADD45α shRNA transfection, HeLa cells were subjected to western blot analysis. b CCK-8 assay showed that silencing of GADD45α significantly prevented HeLa cells from radiation-induced cell death. c Comet assay showed that silencing of GADD45α significantly protected HeLa cells from radiation-induced DNA damage. d Apoptosis analysis showed that silencing of GADD45α significantly protected HeLa cells from radiation-induced apoptosis. e Cell cycle assay showed that silencing of GADD45α significantly protected HeLa cells from radiation-induced G₂/M cell cycle arrest. Each experiment was repeated three times. **p < 0.01; ***p < 0.001