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. 2018 May 9;8:7341. doi: 10.1038/s41598-018-25463-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A consensus phylogenetic tree was constructed using whole mtDNA nucleotide sequence and aa sequences of all 13 PCG combined. Three methods, ML, NJ and ME, were used to build the tree. For the whole mtDNA sequences, the three trees are identical in terms of topology and bootstrap values (all with 100). For aa sequences, the topology is exactly the same as that was built by nt sequences. The bootstrap values are almost the same except for one node that is highlighted in the box. GenBank accession numbers for B. tenagophila (BT) and Radix auricularia are EF433576 and NC_026538, respectively. Please note that due to gene re-arrangement, the gene order of Physella acuta (Physidae) is different from that of Biomphalaria. So mitogenome of Radix auricularia (Lymnaeidae) was used as outgroup.