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. 2018 May 9;8:7349. doi: 10.1038/s41598-018-25799-6

Figure 3.

Figure 3

IL-13 exposure during MTEC differentiation induces goblet cells. (a) Hematoxylin and eosin on tracheal sections of control or house dust mite (HDM) treated mice. Immunofluorescence staining with Mucin 5AC (MUC5AC) shows the presence of goblet cells. MUC5AC expressing cells are indicated by the white arrows. Scale bar, 200 µm and 30 µm. (b) A schematic representation of the culture protocol. (c) Immunofluorescence staining of MUC5AC of MTEC passage 0 and 2 after 8 days of ALI culture with or without IL-13. Scale bar, 30 µM. Graph shows the percentage of MUC5AC expressing goblet cells in culture (mean ± SEM). *p < 0.05 by Student two-tailed t-test (n = 3). (d) Resistance measurements after 8 days of ALI culture with or without IL-13 (5 ng/ml) treatment (mean ± SEM). *p < 0.05 by Student two-tailed t-test (n = 3). Scale bar: 30 µm. (e) Immunofluorescence staining of FOXJ1 positive ciliated cells in a passage 0 (P0) and 2 (P2) after 8 days of ALI culture with or without IL-13 (5 ng/ml). Scale bar, 30 µm. Graph show the percentage of FOXJ1 expressing ciliated cells in culture (mean ± SEM). *p < 0.05 by Student two-tailed t-test (n = 3).