. 2018 May 9;8:7306. doi: 10.1038/s41598-018-25674-4

Table 1.

Identified possible substrates for TG1 using pepK5.

Accession number	Name	Days
Accession number	Name	0	3	7	14
P01029	Complement C4-B		+	+	+
Q8K0E8	Fibrinogen β chain		+	+	+
Q922U2	Keratin, type II cytoskeletal 5		+	+	+
P31725	Protein S100-A9		+	+	+
Q921I1	Serotransferrin		+	+	+
P68373	Tubulin α-1C chain		+	+	+
P99024	Tubulin β-5 chain		+	+	+
P62270	40 S ribosomal protein S18		+	+
Q8R1M2	Histone H2A.J		+	+
Q68FD5	Clathrin heavy chain 1		+		+
O35737	Heterogeneous nuclear ribonucleoprotein H		+		+
P62737	Actin, aortic smooth muscle			+	+
P06909	Complement factor H			+	+
Q8VCM7	Fibrinogen γ chain			+	+
Q9Z2X1	Heterogeneous nuclear ribonucleoprotein F			+	+
Q91X17	Uromodulin			+	+
Q8CGP1	Histone H2B type 1-K		+
P62960	Nuclease-sensitive element-binding protein 1		+
Q9WV32	Actin-related protein 2/3 complex subunit 1B			+
Q8BSL7	ADP-ribosylation factor 2			+
Q8JZQ5	Amiloride-sensitive amine oxidase [copper-containing]			+
P10126	Elongation factor 1-α 1			+
P16110	Galectin-3			+
P11499	Heat shock protein HSP 90-β			+
P02104	Hemoglobin subunit ε-Y2			+
Q99020	Heterogeneous nuclear ribonucleoprotein A/B			+
Q8VEK3	Heterogeneous nuclear ribonucleoprotein U			+
Q8CGP7	Histone H2A type 1-K			+
P10854	Histone H2B type 1-M			+
O89017	Legumain			+
P26041	Moesin			+
P17225	Polypyrimidine tract-binding protein 1			+
Q61656	Probable ATP-dependent RNA helicase DDX5			+
P26043	Radixin			+
Q62093	Splicing factor, arginine/serine-rich 2			+
Q78PY7	Staphylococcal nuclease domain-containing protein 1			+
P26039	Talin-1			+
Q9CWF2	Tubulin β-2B chain			+
P60710	Actin, cytoplasmic 1				+
P63268	Actin, γ-enteric smooth muscle				+
Q60847	Collagen α-1(XII) chain				+
P01942	Hemoglobin subunit α				+
P02089	Hemoglobin subunit β-2				+
P01873	Ig mu chain C region membrane-bound form				+
P08071	Lactotransferrin				+
P11247	Myeloperoxidase				+
P68369	Tubulin α-1A chain				+

Kidney extract on each indicated day after UUO surgery was incubated with biotinylated pepK5. The newly identified possible substrates in each indicated day were demonstrated as “+” compared to control sample (Day 0). The underlined possible substrates indicate overlapped substrates identified as both peptide (pepK5 and pepT26)-incorporated proteins. This Each experiment was done with two replicates from three independent experiments.