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. 2018 May 9;8:7323. doi: 10.1038/s41598-018-25510-9

Figure 4.

Figure 4

Effect of GNE deficiency on IGF-1R downstream signaling pathway: Cells were grown in DCCM, followed by treatment with 5 nM IGF-1 for 10 min or treatment with 5 mM Sialic Acid or 0.5 μM PPP (IGF-1R inhibitor) for 24 h. (A) GNE mutant cells along with r-wt GNE and vector control cell lysates after treatment with or without 5 nM IGF-1 or 5 mM SA were separated on SDS–PAGE and immunoblotted with p-IGF-1R/β-IGF-1R, p-AKT/AKT, BAD/p-BAD, p-ERK/ERK1/2 and GAPDH antibodies. (B) GNE knockdown and vector control cell lysates with or without 5 nM IGF-1 treatment were separated on SDS–PAGE and immunoblotted with p-IGF-1R/β-IGF-1R, p- p-ERK/ERK1/2 and GAPDH antibodies. (C) GNE knockdown and vector control cell lysates after treatment with or without 5 nM IGF-1, 5 mM SA or 0.5 μM PPP (IGF-1R inhibitor) were separated on SDS–PAGE and immunoblotted with p-AKT/AKT, BCL2 and GAPDH antibodies.