Table 1.
Ratio of IGF-1R distribution in various sub-cellular fractions: Ratio of IGF-1R levels in membrane versus cytosolic fraction of various cell lines as calculated by densitometry analysis of Fig. 3 blots.
| Sample | Membrane versus Cytosolic | Membrane fraction | ||
|---|---|---|---|---|
| Untreated | 5 nM IGF-1 | 5 mM SA | Ratio of IGF-1 treated versus Untreated | |
| GNE Knockdown Cells | ||||
| Vector Control | 1.29 | 1.73 | 1.2 | 1.55 |
| GNE Knockdown | 0.75 | 0.93 | 1.77 | 1.25 |
| GNE Mutant Cells | ||||
| Vector Control | 1.09 | 1.42 | 4.26 | 2.32 |
| r- WT GNE | 1.26 | 1.59 | 4.45 | 2.17 |
| r-V603L GNE | 1.24 | 1.58 | 3.44 | 1.96 |
Comparison determines IGF-1R distribution with respect to GNE deficiency. The last column indicate the ratio of IGF-1R distribution in membrane fraction after IGF-1 treatment versus untreated to determine plasma membrane migration pattern.