Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 11;25(5):918–934. doi: 10.1038/s41418-017-0023-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© ADMC Associazione Differenziamento e Morte Cellulare 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — RAB37 promotes formation of the ATG12-ATG5-ATG16L1 complex in a GTP-dependent manner. a–c Gel-filtration analysis of the ATG12-ATG5-ATG16L1 complex by chromatography. Cell lysates of three cell lines (RAB37 overexpression, RAB37 knockdown and control HeLa cells) and two transiently transfected cells (FLAG-RAB37-Q89L and FLAG-RAB37-T43N) were separated by size-exclusion chromatography. Fractions were subjected to Western blotting with anti-ATG16L1 (a), anti-ATG5 (b) or anti-RAB37 antibody (c), respectively. The positions of the molecular mass standards in chromatography are showed in arrows. d Work model of RAB37 function together with ATG5-12 and ATG16L1 for autophagy