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. 2018 May 9;8:7367. doi: 10.1038/s41598-018-25668-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamic localization of RBM3 in differentiating primary myoblasts. (a) Image of F-actin (red, phalloidin), RBM3 (green) in myoblasts fixed shortly after plating (1 hr) onto fibronectin. SICs are clearly visible along the edges of recently adherent cells as F-actin-delimited outpouchings of membrane. RBM3 is concentrated within SICs. (c) DAPI-stained nuclei in cells from Panel a. (b and d) Blowup of upper and lower margins of cell from the top-right portion of panel A. SICs (arrowheads) can be seen clearly with a peripheral F-actin ring surrounding an RBM3-rich core. (e) Image of a myoblasts partially differentiated into a myotube. At this stage, RBM3 is present in membranous blebs devoid of F-actin that are known to mediate myoblast migration. (f) Image of a fully differentiated satellite cell. RBM3 relocalizes from membrane protrusions to the cytoplasm and nucleus (nuc). (g) Images of a myotube explant at 24 hrs in culture in which activated satellite cells migrating along the myotube border (dashed outline) co-express MyoD (red) and RBM3 (green). (h) Images of dividing satellite cells along the border of a myotube that co-label for histone H3 phosphate (H3-P, red) and RBM3 (green).